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Evaluation of Exon Inclusion Induced by Splice Switching Antisense Oligonucleotides in SMA Patient Fibroblasts
07:02

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Published on: May 11, 2018

[Antisense oligonucleotide targeting survivin affects the proliferation and apoptosis of SMMC-7721 cells]

Kejian Pan1, Xiaojing Liu, Chunlei Yang

  • 1Division of Basic Medical Science, Chengdu Medical College, Chengdu 610083, China. pkj6696@yahoo.com.cn

Sheng Wu Yi Xue Gong Cheng Xue Za Zhi = Journal of Biomedical Engineering = Shengwu Yixue Gongchengxue Zazhi
|July 22, 2006
PubMed

Insights

Antisense oligonucleotides targeting survivin effectively reduced cancer cell proliferation and increased apoptosis. This suggests a potential for selective tumor therapy in the future.

Area of Science:

  • Molecular Biology
  • Cancer Research
  • Apoptosis Regulation

Background:

  • Survivin, an inhibitor of apoptosis (IAP) protein, is highly expressed in various human cancers but not in normal adult tissues.
  • This differential expression makes survivin a promising target for selective cancer therapies.

Purpose of the Study:

  • To design and evaluate the efficacy of a human survivin antisense oligonucleotide.
  • To assess the impact of survivin inhibition on cancer cell proliferation and apoptosis.

Main Methods:

  • Reverse Transcription Polymerase Chain Reaction (RT-PCR) and Western blot to confirm survivin mRNA and protein reduction.
  • MTT assay to measure SMMC-7721 cell proliferation inhibition.
  • Flow cytometry and TUNEL assay to quantify apoptosis rates.

Main Results:

  • The survivin antisense oligonucleotide significantly decreased survivin mRNA and protein levels in SMMC-7721 cells.
  • Cell proliferation was inhibited by 43.28% in the antisense oligonucleotide group compared to 6.76% in the control.
  • Apoptosis rates increased to 33.68% with antisense oligonucleotide treatment, significantly higher than the control group's 14.12%.

Conclusions:

  • Human survivin antisense oligonucleotides effectively inhibit cancer cell proliferation and induce apoptosis.
  • Survivin-targeted antisense oligonucleotides demonstrate potential as a selective therapeutic strategy for human cancers.

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