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[Determination of alkaloids in Radix Aconiti Lateralis Preparata by RP-ion-pair HPLC]
1West China School of Pharmacy, Sichuan University, Chengdu.
Aim:
To separate and quantitatively determine six alkaloids: aconitine, mesaconitine, hypaconitine, beiwutine, benzoylaconine and benzoylmesaconine in the Chinese traditional medicine Radix Aconiti Lateralis Preparata (Fuzi).
Methods:
A RP-ion-pair HPLC method was established. An AichromBond-1 C18 column was used at a column-temperature of 35 degrees C. The mobile phase was CH3CN5 mmol x L(-1) NaH2PO4(50:50) containing 7 mmol x L(-1) SDS at a flow-rate of 1.0 mL x min(-1). The detector was set at UV 235 nm.
Results:
These six alkaloids can be completely separated and determined quantitatively.
Conclusion:
This method is accurate and suitable for the determination of six alkaloids in Fuzi.
