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Updated: Jul 21, 2026

Amide Hydrogen/Deuterium Exchange & MALDI-TOF Mass Spectrometry Analysis of Pak2 Activation
Published on: November 26, 2011
Peptides for disruption of PKA anchoring
C Hundsrucker1, W Rosenthal, E Klussmann
1Leibniz-Institut für Molekulare Pharmakologie, Campus Berlin-Buch, Robert-Rössle-Strasse 10, D-13125 Berlin, Germany.
Abstract:
Adaptor or scaffolding proteins are at the basis of multiprotein complexes that spatially and temporally co-ordinate the propagation and integration of a broad range of cellular events. One class of scaffolding proteins are AKAPs (A-kinase-anchoring proteins). They sequester PKA (protein kinase A) and other signalling molecules including phosphodiesterases, other protein kinases and protein phosphatases to specific subcellular compartments. AKAP-dependent protein-protein interactions play a role in many physiologically relevant processes. For example, AKAP-PKA interactions are essential for the vasopressin-mediated water re-absorption in renal collecting duct principal cells or beta-adrenoceptor-induced increases in cardiac myocyte contractility. Here, we discuss recently developed peptide disruptors of AKAP-PKA interactions. Such peptides are valuable tools to study the relevance of PKA anchoring in cellular processes.
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