Characteristics and kinetics of proopiomelanocortin mRNA expression by human leucocytes

Northern blot analysis of total and poly(A)+ RNA demonstrated that human leucocytes contain several proopiomelanocortin (POMC) mRNA species, including 0.8-, 1.2-, 1.5-, and 9.5-kb transcripts. Peripheral blood lymphocytes (PBL) were found to express all four species. The other cell types had either the 0.8-kb species alone or both 0.8- and 9.5-kb species. Neutrophils were the only cells to express the 9.5-kb transcript alone. In T cell clones, both interleukin (IL)-2 alone or the antigen for which the clone was specific induced POMC accumulation within 18-24 h. Cytoplasmic dot blot analysis of PBL RNA demonstrated that POMC expression could be induced by corticotrophin releasing factor, rIL-1, and phorbol ester, but not by calcium ionophore (A23187). In PBL activated in a mixed lymphocyte culture there was increased expression, particularly of the smaller species (0.8, 1.2, and 1.5 kb), within 1-3 days of activation. The intensity of the bands began to decline thereafter. The 9.5-kb POMC transcript essentially disappeared by Day 3.