Biochemically differentiated mouse glial lines carrying a nervous system specific cell surface antigen (NS-1)

Area of Science:

• Neuroscience
• Oncology
• Cell Biology

Background:

• Established six biochemically differentiated clonal lines from a transplantable mouse glioma (tg26).
• Previously raised antibodies against G26 tumor cells defining nervous system antigen-1 (NS-1).
• NS-1 is a cell surface component exclusively found in the nervous system.

Purpose of the Study:

• Characterize biochemical and antigenic properties of the established glioma clonal lines.
• Investigate the regulation of NS-1 expression in vitro and in vivo.
• Compare the regulation of NS-1 with other markers like H-2 histocompatibility antigen, S-100 protein, and 2:3-cyclic nucleotide 3-phosphohydrolase.

Main Methods:

• Established and cultured six clonal glioma lines from C57BL/6 mouse strain.
• Measured NS-1 concentrations in vitro and in vivo.
• Assessed the effect of dibutyryl cyclic AMP on NS-1 and H-2 antigen levels.
• Analyzed the expression of S-100 protein and 2:3-cyclic nucleotide 3-phosphohydrolase.
• Evaluated cellular response to catecholamines.

Main Results:

• NS-1 concentrations were high in vivo but reduced in vitro, further decreasing with dibutyryl cyclic AMP.
• H-2 histocompatibility antigen concentration remained unaffected by dibutyryl cyclic AMP.
• Cell lines synthesized S-100 protein (high in vitro, negligible in vivo) and had high 2:3-cyclic nucleotide 3-phosphohydrolase activity (undetectable in vitro, detectable in vivo).
• Cells responded to catecholamines by increasing intracellular cyclic AMP levels.

Conclusions:

• Glioma clonal lines express nervous system-specific markers (NS-1, S-100, 2:3-cyclic nucleotide 3-phosphohydrolase).
• Expression of membrane-bound NS-1 and 2:3-cyclic nucleotide 3-phosphohydrolase is differentially regulated compared to soluble S-100 protein.
• Dibutyryl cyclic AMP affects NS-1 levels but not H-2 antigen levels, suggesting distinct regulatory mechanisms.