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Related Experiment Videos

Thin-foil magnetic force system for high-numerical-aperture microscopy.

J K Fisher1, J Cribb, K V Desai

  • 1Department of Biomedical Engineering, University of North Carolina, Chapel Hill, North Carolina 27599-7575.

The Review of Scientific Instruments
|July 22, 2006
PubMed
Summary

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We developed a versatile magnetic manipulation system for applying precise forces in biological studies. This system enhances high-numerical-aperture microscopy applications, enabling new insights into force-dependent biological processes.

Area of Science:

  • Biophysics
  • Microbiology
  • Cell Biology

Background:

  • Forces are crucial in biological processes from protein dynamics to cell division.
  • Existing force application methods have limitations in force range or compatibility with high-NA microscopy.
  • Magnetic manipulators offer potential but are often bulky, hindering combined applications.

Purpose of the Study:

  • To develop a novel magnetic manipulation system for precise force application in biological research.
  • To overcome limitations of existing methods by combining high force application with high-numerical-aperture microscopy.
  • To investigate the role of forces in higher-order chromosome structure and function.

Main Methods:

  • Designed a magnetic manipulation system with removable pole plates for adaptable magnetic field geometry.

Related Experiment Videos

  • Achieved force application exceeding 700 pN (1 µm particle) and 13 nN (4.5 µm particle).
  • Integrated a feedback-enhanced laser tracking system (2.4 nm resolution, 10 kHz bandwidth).

    • Main Results:

    • The system is compatible with commercial high-numerical-aperture microscope objectives.
    • Demonstrated forces over the full 4π steradians with a bandwidth exceeding 3 kHz.
    • Successfully applied the system to study force-dependent higher-order chromosome structure and function.

    Conclusions:

    • The developed magnetic manipulation system offers a versatile and powerful tool for biological force studies.
    • Its compatibility with high-NA microscopy opens new avenues for investigating force-dependent biological phenomena.
    • The system provides high force, high bandwidth, and high resolution for advanced microbiological applications.