[Sorcin overexpression and multidrug resistance in human gastric cancer cells]
Hong Yi1, Yi-xuan Yang, Can-e Tang
1Key Laboratory of Cancer Proteomics of Ministry of Health of China, Xiangya Hospital, Central South University, Changsha.
Objective:
To screen multidrug resistance (MDR) related proteins in human gastric cancer using proteomics technique.
Methods:
Two-dimensional electrophoresis (2-DE) was used to separate the total proteins of vincristine-resistant human gastric cancer cell line SGC7901/VCR and its counterpart SGC7901. PDQuest software was used to analyze 2-DE images, and the differential expression proteins between the 2 cell lines were identified by both MALDI-TOF-MS and ESI-Q-TOF. The differential expression level of sorcin, one of the identified proteins, was confirmed by western blot analysis. The effect of sorcin on the development of MDR of SGC7901/VCR was determined by antisense oligonucleotides (ASO) technique.
Results:
Sorcin as a high expression protein in SGC7901/VCR was identified and the suppression of sorcin expression by sorcin ASO could enhance the vincristine chemosensitivity in SGC7901/VCR.
Conclusion:
Sorcin overexpression is related to MDR in human gastric cancer cell line SGC7901/VCR.
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