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Cyclodextrin-insulin complex encapsulated polymethacrylic acid based nanoparticles for oral insulin delivery
1Division of Biosurface Technology, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Thiruvananthapuram 695012, Kerala, India.
International Journal of Pharmaceutics
|July 25, 2006
Summary
Developing an oral insulin delivery system using hydroxypropyl beta cyclodextrin-insulin (HPbetaCD-I) complex encapsulated polymethacrylic acid-chitosan-polyether (PMCP) nanoparticles shows promise for effective and biologically active insulin delivery.
Area of Science:
- Biomaterials Science
- Drug Delivery Systems
- Nanotechnology
Background:
- Oral insulin delivery remains a significant challenge due to insulin's degradation in the gastrointestinal tract.
- Conventional insulin administration requires injections, leading to patient discomfort and compliance issues.
- Developing effective oral insulin formulations is crucial for improving diabetes management.
Purpose of the Study:
- To develop and characterize novel mucoadhesive nanoparticles for oral insulin delivery.
- To encapsulate hydroxypropyl beta cyclodextrin-insulin (HPbetaCD-I) complex within polymethacrylic acid-chitosan-polyether (PMCP) nanoparticles.
- To evaluate the in vitro release, biological activity, and mucoadhesive properties of the developed nanoparticles.
Main Methods:
- Nanoparticles were synthesized using free radical polymerization of methacrylic acid with chitosan and polyether.
- Insulin was complexed with hydroxypropyl beta cyclodextrin (HPbetaCD) and encapsulated into PMCP nanoparticles via diffusion filling.
- Particle size was analyzed using Dynamic Light Scattering (DLS); complexation was confirmed by FTIR and fluorescence spectroscopy.
- In vitro release studies were conducted at varying pH; biological activity was assessed using ELISA; mucoadhesion was tested on rat intestinal mucosa.
Main Results:
- PMCP nanoparticles exhibited a size distribution between 500-800 nm.
- High insulin encapsulation efficiency was achieved, with release profiles dependent on pH.
- Encapsulated insulin retained biological activity as confirmed by ELISA.
- PMCP nanoparticles demonstrated fair mucoadhesive properties, indicating potential for oral absorption.
Conclusions:
- HPbetaCD-I complex encapsulated PMCP nanoparticles represent a promising strategy for oral insulin delivery.
- The mucoadhesive and pH-responsive nature of the nanoparticles facilitates effective insulin delivery.
- This novel system offers a potential non-invasive alternative to insulin injections for diabetes treatment.
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