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Real-time Monitoring of Ligand-receptor Interactions with Fluorescence Resonance Energy Transfer
Published on: August 20, 2012
Fluorescence quenching of water-soluble conjugated polymer by metal cations and its application in sensor
Yan-Guo Chen1, Dan Zhao, Zhi-Ke He
1College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, PR China.
Abstract:
The effects of different metal cations on the fluorescence of water-soluble conjugated polymer (CP) and their quenching mechanism have been explored. Most transition metal cations, especially noble metal cations, such as Pd2+, Ru3+, and Pt2+ possessed higher quenching efficiency to CP fluorescence than that of the main group metal cations and other transition metal cations, which have filled or half-full outmost electron layer configurations. Base on this, rapid, sensitive detection of noble metal cations can be realized and a novel quencher-tether-ligand (QTL) probe was developed to detect avidin and streptavidin.

