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Vaccinia Virus Infection & Temporal Analysis of Virus Gene Expression: Part 1
12:00

Vaccinia Virus Infection & Temporal Analysis of Virus Gene Expression: Part 1

Published on: April 8, 2009

Adenovirus-induced alterations in host cell gene expression prior to the onset of viral gene expression

Fredrik Granberg1, Catharina Svensson, Ulf Pettersson

  • 1Department of Genetics and Pathology, Rudbeck Laboratory, Uppsala University, SE-751 85 Uppsala, Sweden.

Virology
|July 25, 2006
PubMed

Insights

Adenovirus infection rapidly triggers gene expression changes in lung cells, initially inhibiting cell growth and initiating an immune response. These early gene responses are temporary, ceasing once viral early gene expression begins.

Area of Science:

  • Molecular biology
  • Virology
  • Cellular biology

Background:

  • Adenovirus infections can significantly impact host cell functions.
  • Understanding the early molecular events following viral entry is crucial for comprehending host-pathogen interactions.

Purpose of the Study:

  • To investigate the gene expression profiles in human lung fibroblasts during the initial stages of adenovirus infection.
  • To identify key cellular processes affected by adenovirus during the immediate-early phase.

Main Methods:

  • Gene expression profiling of human primary lung fibroblasts (IMR-90).
  • Time course analysis to observe changes in gene expression post-adenovirus infection.

Main Results:

  • Eight of twelve studied genes encoded transcription factors involved in cell growth inhibition (e.g., ATF3, KLF4) and immune response (e.g., NR4A1, CEBPB).
  • These immediate-early response genes showed transient induction.
  • Gene expression changes were suppressed after the onset of adenovirus early gene expression.

Conclusions:

  • Early adenovirus infection induces a transient growth arrest and immune response in lung fibroblasts.
  • The host cell's immediate-early gene response is regulated and suppressed by the virus during its replication cycle.

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