Surface coat synthesis and turnover from epimastigote to bloodstream forms of Trypanosoma brucei

Monoclonal antibodies to metacyclic surface coat glycoproteins of Trypanosoma brucei brucei STIB 247LG were produced for a study of the synthesis of metacyclic variable surface glycoproteins (VSGs) within the salivary gland of Glossina morsitans morsitans, and of the first exchange of the surface glycoproteins after infection in mice. Immunofluorescence antibody tests and protein A-gold labelling revealed that the VSGs are continuously integrated into the whole surface of the trypanosome while it is still attached to the gland epithelium. A pool of 8 antibodies recognized about 50% of the metacyclic forms present in the saliva of an infected tsetse fly, which confirmed the heterogeneity of the metacyclic VSG-generation. The labelling experiments showed that the integration of the first VSG-generation into the surface of bloodstream forms takes place in the same way as in the metacyclics. This process started on day 3 after infection and was finished on day 6.