Related Experiment Video
Updated: Aug 7, 2026

DNA Sequence Recognition by DNA Primase Using High-Throughput Primase Profiling
Published on: October 8, 2019
PRINS for the detection of unique sequences
Stephen S Wachtel1, Avirachan T Tharapel
1Department of Obstetrics and Gynecology, University of Tennessee, Memphis, TN, USA.
Abstract:
Primed in situ labeling (PRINS) can be used to localize single-copy genes and unique sequences. Using a modified PRINS method that incorporates multiple primers for the same sequence, single-step annealing and extension, anti-Taq DNA polymerase antibody, and stringent washing, we localized the human SRY gene to Yp11.31-p11.32 in chromosome preparations in situ. Locus-specific oligonucleotide probes (i.e., PRINS primers) were annealed to chromosomal DNA fixed on glass slides and extended in the presence of the four trinucleotide precursors, biotin-16-2'-deoxyuridine 5'-triphosphate, Tris-HCl, KCl, MgCl2, bovine serum albumin, and Taq DNA polymerase. After reaction with avidin-conjugated fluorophores, the resulting signals could be visualized by fluorescence microscopy in metaphase spreads and in interphase nuclei. This method could prove useful for unique sequences in general.
Related Concept Videos
Sanger Sequencing
Next-generation Sequencing
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.
RNA-seq
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...

