Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cross-talk between APC/C and CBP/p300.

Jianghuai Liu1, Serge Y Fuchs

  • 1Department of Animal Biology, University of Pennsylvania, Philadelphia, Pennsylvania 19104-6046, USA. jiangliu@vet.upenn.edu

Cancer Biology & Therapy
|July 25, 2006
PubMed
Summary

The Anaphase-Promoting Complex/Cyclosome (APC/C) interacts with CBP and p300, revealing new roles in regulating both cell cycle and gene transcription.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

In vivo CRISPR knockout screen identifies Polr1a as a key driver and a potential therapeutic target for melanoma metastasis.

Oncogene·2026
Same author

Mono-ADP-ribosylation-driven immunosuppression and cross-resistance to therapy through cancer cell intrinsic and extrinsic mechanisms.

bioRxiv : the preprint server for biology·2026
Same author

Optimization of IS621 recombinase/bridge RNA-directed recombination for precise insertion of large DNA fragments in human cells.

Nature communications·2026
Same author

SPTBN2 promotes an immunosuppressive tumor microenvironment and cross-resistance to anti-cancer therapies.

bioRxiv : the preprint server for biology·2026
Same author

Noncanonical target-strand cytosine base editing via engineered Un1Cas12f1 platform.

Nature communications·2025
Same author

Configuration of adaptable template RNA architectures to unfold the editable space of a nuclease prime editor.

Nucleic acids research·2025

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biochemistry

Background:

  • The Anaphase-Promoting Complex/Cyclosome (APC/C) is a critical regulator of cell cycle progression.
  • APC/C functions as a ubiquitin E3 ligase, targeting key cell cycle proteins for degradation.
  • The transcription co-activators CBP and p300 are known for their roles in gene expression regulation.

Purpose of the Study:

  • To investigate the interaction between the APC/C complex and the transcription co-activators CBP and p300.
  • To elucidate the functional significance of this interaction in cellular processes.

Main Methods:

  • Co-immunoprecipitation assays to detect APC/C-CBP/p300 interactions.
  • Analysis of APC/C subunits (APC5 and APC7) involved in the interaction.
  • Functional assays to assess the impact on transcription and cell cycle progression.

Main Results:

  • Demonstrated a direct interaction between the APC/C complex and CBP/p300.
  • Identified APC5 and APC7 as the specific APC/C subunits mediating this interaction.
  • Showcased the functional importance of the APC/C-CBP/p300 interaction in regulating both gene transcription and cell cycle progression.

Conclusions:

  • The APC/C complex engages with transcription co-activators CBP and p300.
  • This interaction integrates cell cycle control with transcriptional regulation.
  • Findings suggest novel, dual roles for APC/C, CBP, and p300 in cellular regulation.

Related Experiment Videos