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Updated: Aug 7, 2026
Bimolecular Fluorescence Complementation
Published on: April 15, 2011
An improved mRFP1 adds red to bimolecular fluorescence complementation
Guido Jach1, Martina Pesch, Klaus Richter
1Max Planck Institute for Plant Breeding Research, Department of Plant Developmental Biology, Carl von Linne Weg 10, D-50829 Cologne, Germany. jach@mpiz-koeln.mpg.de
Abstract:
Protein-protein interactions are fundamental to virtually every aspect of cellular functions. Blue, green and yellow bimolecular fluorescence complementation (BiFC) systems based on GFP and its variants allow the investigation of protein-protein interactions in vivo. We have developed the first red BiFC system based on an improved monomeric red fluorescent protein (mRFP1-Q66T), expanding the range of possible applications for BiFC.
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