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Updated: Aug 7, 2026
Chromosome Screening of Human Preimplantation Embryos by Using Spent Culture Medium: Sample Collection and Chromosomal Ploidy Analysis
Published on: September 7, 2021
Chromosome analysis of uncultured amniocytes by comparative genomic hybridization in early amniocentesis
Aphornpirom Ketupanya1, Niwat Aranyakasemsuk, Chintana Tocharoentanaphol
1Department of Obstetrics, Phramongkutklao Hospital, Bangkok, Thailand. chrodna@yahoo.com
Objective:
To study chromosome analysis by comparative genomic hybridization (CGH) compared with the conventional technique in early amniocentesis.
Material And Method:
Cross-sectional descriptive study design was performed in 32 singleton pregnant women with gestational age between 12-15 weeks. Transabdominal amniocentesis was carried out under ultrasound guidance. The amniotic fluid samples were simultaneously investigated using CGH and the conventional cytogenetics study as a gold standard.
Results:
Amniocentesis were done for advanced maternal age in all cases. The mean maternal age was 35.8 years (35-42 years). The mean gestational age was 13.7 weeks (12-15 weeks). The chromosome analysis by CGH technique of uncultured amniocyte showed 17 normal female chromosomes (53.1%) and 15 normal male chromosomes (46.9%). This finding was the same as the conventional cytogenetics method. The mean duration of the CGH method was 6 days and that of the conventional cytogenetics method was 13.7 days (10-23 days).
Conclusion:
The CGH technique is a reliable technique for a rapid prenatal diagnosis of chromosome study in early gestation.
