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Updated: Aug 7, 2026

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Optimized PCR-based Detection of Mycoplasma
Published on: June 20, 2011
[Detection of Mycoplasma hominis in Trichomonas vaginalis by PCR]
Shun-li Fang1, Jian-chun Xiao, Zhao-rong Lun
1Center for Parasitic Organisms, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China.
Abstract:
Twelve of 28 isolates of Trichomonas vaginalis collected from patients in hospitals in Guangzhou were found naturally infected with Mycoplasma hominis by PCR using specific primers. It suggests that the symbiosis of M. hominis in T. vaginalis also commonly occurs in China.
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Trichomoniasis
Trichomonas vaginalis is a flagellated protozoan parasite and the causative agent of trichomoniasis, one of the most prevalent non-viral sexually transmitted infections in the United States. This extracellular parasite primarily colonizes the lower genitourinary tract in women—particularly the vagina—and in men, the urethra and prostate. Its structural and functional adaptations enable its survival, motility, and pathogenicity within the host environment.Structural Features and Host EntryT.
PCR
Overview
Real Time RT-PCR
Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
The real-time quantification of the number of amplified products is...
