[Flow cytometry analysis of leukocytes response in rats immunized and infected with Fasciola hepatica]

Flow cytometry analysis of leukocytes response in rats immunized and infected with Fasciola hepatica. In the present experiment the differences in blood leucocytes between rats immunised intranasally with cDNA encoding F. hepatica GST, challenged with the parasite (group 1) and non-immunised infected rats (group 2) were compared. The number of leukocytes were estimated by flow cytometry on the 1, 5, 9 week after infection. Changes in the level of blood lymphocytes and monocytes were similar, with an increasing tendency in both groups. The level of eosinophils decreased to the 9th week after infection in both groups, however the number of these cells was seven times higher in control rats than in immunised rats.