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Updated: Aug 6, 2026

Chromatin Immunoprecipitation (ChIP) to Assay Dynamic Histone Modification in Activated Gene Expression in Human Cells
Published on: July 29, 2010
[The relationship between histone acetylation modification and IFN-gamma responsive gene regulation]
Jin-jun Guo1, Ying Huang, Jun Zhang
1Key Laboratory of Molecular Biology of Infectious Diseases, Ministry of Education, Institute for Viral Hepatitis, Chongqing University of Medical Sciences, Chongqing 400010, China.
Objectives:
To study the role of histone modification in the regulation of IFN-gamma-activated gene using chromatin immunoprecipitation technique.
Methods:
Real-time PCR was used to measure the mRNA level of interferon-gamma-inducible protein 10 (IP-10) in Hela cells. Chromatin immunoprecipitation combined with Real-time PCR was used to check the histone H4 acetylation level at IFN-stimulated response element (ISRE) locus of IP-10 gene.
Results:
IP-10 was strongly activated by IFN-gamma. The histone H4 deacetylation happened at the ISRE locus when IP-10 was induced by IFN-gamma. The activation of IP-10 and the deacetylation of histone H4 at the ISRE site induced by IFN-gamma were inhibited or blocked by histone deacetylase (HDAC) inhibitor trichostatin A (TSA).
Conclusion:
The histone H4 deacetylation at the ISRE site is related with the activation of IP-10 by IFN-gamma.
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