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Digital techniques for luminescence detection in liquid chromatography with an intensified linear photodiode array
B J Clark1, A F Fell, D G Jones
1Department of Pharmaceutical Chemistry, University of Bradford, Bradford BD 7 1DP, UK.
Journal of Pharmaceutical and Biomedical Analysis
|January 1, 1988
Summary
This study explores a rapid-scanning fluorescence detector for liquid chromatography (LC) in pharmaceutical analysis. It assesses peak homogeneity using spectral normalization, differentiation, and ratio chromatograms for drug impurities.
Area of Science:
- Analytical Chemistry
- Chromatography
- Spectroscopy
Background:
- Rapid-scanning fluorescence detectors offer advanced capabilities for analyzing complex mixtures.
- Intensified linear photodiode array detectors provide high-sensitivity detection in liquid chromatography (LC).
- Pharmaceutical and biomedical analyses require precise methods for compound identification and purity assessment.
Purpose of the Study:
- To evaluate the utility of a rapid-scanning fluorescence detector in LC for pharmaceutical and biomedical applications.
- To demonstrate techniques for assessing chromatographic peak homogeneity using this detector technology.
- To showcase the detector's application in analyzing polynuclear aromatic hydrocarbons and atenolol impurities.
Main Methods:
- Utilizing an intensified linear photodiode array detector for data acquisition.
- Generating isometric plots of (I(em),lambda(em),t) at defined excitation wavelengths.
- Applying spectral normalization, second-order differentiation, and fluorescence emission ratio chromatograms for peak analysis.
Main Results:
- Demonstrated the effectiveness of peak homogeneity assessment techniques.
- Successfully analyzed polynuclear aromatic hydrocarbons and atenolol with its impurities.
- Provided a comprehensive overview of the detector's capabilities and data presentation.
Conclusions:
- The rapid-scanning fluorescence detector shows significant potential for pharmaceutical and biomedical analysis.
- Advanced data processing techniques enhance the assessment of chromatographic peak purity.
- This technology offers promising avenues for future developments in LC detection.
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