Related Experiment Video
Updated: Aug 6, 2026

Optical Photothermal Infrared-Fluorescence In Situ Hybridization (OPTIR-FISH)
Published on: February 23, 2024
Analysis of 5-OH-indoles in human gut biopsy tissues by reversed-phase high-performance liquid chromatography with
B Viell1, K H Vestweber, B Krause
12nd Department of Surgery, University of Cologne, Ostmerheimer Strasse 200, D-5000 Köln 91, FRG.
Abstract:
A method was devised for the rapid simultaneous determination of major indoles in human gut tissues. Analysis with picomol detection limits was done by HPLC on a C(18) reversed-phase column with fluorimetric detection at 276/350 nm. This simple method for which there is no necessity of derivatization or purification was validated for routine analysis of small mucosa samples (less than 4 mg fresh weight) obtainable during endoscopy. A comprehensive list of 5-OH-indole compounds in human gut tissue is presented.

