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Updated: Aug 6, 2026

Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns
Published on: April 26, 2016
Analysis of some dosage forms containing pyridine derivatives using a cyclodextrin bonded stationary phase in HPLC
S El Gezawi1, N Omar, N El Rabbat
1Department of Pharmacy, University of Assiut, Assiut Egypt.
Abstract:
The high performance liquid chromatography of some pyridine derivatives using a silica column to which beta-cyclodextrin has been bonded, has been investigated. In spite of the low affinity constants of the drugs for cyclodextrin (of the order of 10(2) M(-1)) good separations can be achieved using a mobile phase of methanol and pH 7.0 phosphate. Good analysis of some dosage forms are achieved, including one on a tablet containing pheniramine maleate, pyrilamine maleate and phenylpropanolamine hydrochloride. Extractions and chromatography are quick and simple.
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