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Problems with the immunoassay of digoxin

L Molin1, B Bergdahl, G Dahlström

  • 1Natl Lab Forensic Chemistry Department of Toxicology, University Hospital, S-581 85 Linköping, Sweden.

Journal of Pharmaceutical and Biomedical Analysis
|January 1, 1987
PubMed

Factors that affect the standardization and reliability of the radioimmunoassay of digoxin are reviewed. Some new data are presented on standardization and suggestions are made for dealing with problems in the design and techniques of assays.

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In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.