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Partial development of a radioimmunoassay for horse metallothionein
C Hogstrand1, P E Olsson, C Haux
1Department of Zoophysiology, University of Göteborg, P.O. Box 25059, S-400 31 Göteborg, Sweden.
Abstract:
The partial development and evaluation of a radioimmunoassay for horse metallothionein (MT) with low amounts of antigen is described. Factors that affect the yield of the conjugation reaction between horse MT and bovine IgG are discussed. Dot-blot has been used as a simple, rapid and inexpensive test for antibody screening.
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