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Updated: Aug 6, 2026

Generation of Murine Monoclonal Antibodies by Hybridoma Technology
09:42

Generation of Murine Monoclonal Antibodies by Hybridoma Technology

Published on: January 2, 2017

Development of in vitro immunization in murine and human hybridoma technology

C A Borrebaeck1

  • 1Department of Biotechnology, University of Lund, P.O. Box 124, S-221 00 Lund, Sweden.

Journal of Pharmaceutical and Biomedical Analysis
|January 1, 1987
PubMed

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Hybridoma Technology01:31

Hybridoma Technology

Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation, polyethylene glycol...