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Updated: Aug 6, 2026

Homogeneous Time-resolved Förster Resonance Energy Transfer-based Assay for Detection of Insulin Secretion
Published on: May 10, 2018
HPLC as a replacement for the animal response assays for insulin
1Central Analytical Laboratories (Chemical), The Wellcome Foundation Ltd, Temple Hill, Dartford, Kent DA1 5AH, UK.
Abstract:
The work describes the assay of the potency of insulin (drug substance) and its formulations by gradient elution reversed-phase high-performance liquid chromatography (HPLC). The methods are shown to differentiate between insulins of bovine, porcine and human sequence, and to be both reproducible and stability-indicating. The HPLC assay results show good agreement with those obtained by the mouse blood glucose assay. The advantages of the HPLC assays over the animal response assays are discussed. It is suggested that the animal response assays should now be replaced by the HPLC assays.
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