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Determination of ibuprofen in human plasma by solid phase extraction and reversed-phase high-performance liquid
J H Jonkman1, R Schoenmaker, A H Holtkamp
1Pharma Bio-Research International B. V., Department of Biopharmaceutics and Clinical Pharmacokinetics, P.O. Box 147, 9400 AC Assen, The Netherlands.
Abstract:
A new, sensitive and simple method for the rapid quantitative determination of ibuprofen in human plasma has been developed. This method involves the use of a solid phase extraction on "Baker" C-18 disposable extraction columns for sample clean-up and uses mefenamic acid as an internal standard. Separation and quantitation are performed by reversed-phase liquid chromatography using a Nucleosil C18 column and methanol-0.04 M phosphoric acid (80:20, v/v), as the mobile phase. Detection was achieved by UV-absorbance measurements at 229 nm.
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