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Determination of amoxycillin in plasma by ion pair column extraction and reversed-phase ion pair high-performance
J H Jonkman1, R Schoenmaker, J Hempenius
1Pharma Bio-Research Int., Laboratory for Drug Analysis and Biopharmaceutics, P.O. Box 147, 9400 AC Assen, The Netherlands; State University, Department of Pharmaceutical and Analytical Chemistry, Ant. Deusinglaan 2, 9713 AW Groningen, The Netherlands.
Abstract:
A quantitative method is described for the determination of amoxycillin in plasma. The method utilizes ion pair extraction of amoxycillin on disposable columns packed with Baker-10 SPE octadecyl, with tetrabutylammonium ion as the counter ion and methanol as the eluent. Separation and quantitation is performed by reversed-phase ion pair high-performance liquid chromatography (Nucleosil C-18) using the same counter ion and a mobile phase of methanol-phosphate buffer (pH 6.0) (31:69 v/v) with detection at 229 nm.
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