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A simple high-performance liquid chromatographic method for estimating human serum angiotensin-converting enzyme
V Maurich1, M Moneghini, A Pitotti
1Istituto di Tecnica Farmaceutica, Università degli Studi di Trieste, 34139 Trieste, Italy.
Journal of Pharmaceutical and Biomedical Analysis
|January 1, 1985
Summary
A new, simple method accurately measures angiotensin-converting enzyme activity in human serum. This technique uses high-performance liquid chromatography to quantify liberated hippuric acid, aiding in clinical diagnostics.
Area of Science:
- Biochemistry
- Analytical Chemistry
- Clinical Diagnostics
Background:
- Angiotensin-converting enzyme (ACE) plays a crucial role in the renin-angiotensin system.
- Accurate measurement of ACE activity is important for diagnosing and managing cardiovascular and renal diseases.
Purpose of the Study:
- To develop a simple and direct method for quantifying angiotensin-converting enzyme activity in human serum.
Main Methods:
- Serum samples were incubated with the substrate hippurylhistidylleucine.
- Liberated hippuric acid was quantified using reversed-phase ion-pair high-performance liquid chromatography (RP-IP-HPLC).
- UV spectrometric detection was employed for accurate measurement.
Main Results:
- The developed method provides a straightforward approach to measuring ACE activity.
- RP-IP-HPLC with UV detection offers reliable quantification of the enzymatic product.
Conclusions:
- This method offers a simple, effective, and direct way to determine serum ACE activity.
- The technique is suitable for routine clinical laboratory use and research applications.
