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Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns
Published on: April 26, 2016
New techniques for liquid chromatography in open-tubular columns
J W Jorgenson1, E J Guthrie, R L St Claire
1Department of Chemistry, University of North Carolina, Chapel Hill, NC 27514, USA.
Journal of Pharmaceutical and Biomedical Analysis
|January 1, 1984
Summary
This study introduces a novel cross-linked polymer stationary phase for capillary chromatography, enabling effective separation of urinary components. The new method utilizes advanced detection techniques for enhanced analysis.
Area of Science:
- Analytical Chemistry
- Separation Science
- Chromatography
Background:
- Capillary chromatography is crucial for analyzing complex biological samples.
- Development of advanced stationary phases is key to improving separation efficiency.
- Existing methods require optimization for sensitive detection of urinary components.
Purpose of the Study:
- To describe methods for sample introduction, capillary column fabrication, and on-column detection.
- To introduce a new cross-linked polymer stationary phase coating for capillary chromatography.
- To demonstrate the separation of urinary components using this new column technology.
Main Methods:
- Fabrication of 16-micrometer open-tubular capillary columns.
- Application of a novel cross-linked polymer stationary phase coating.
- Utilizing on-column electrochemical detection (ECD) and laser-induced fluorescence detection (LIFD).
Main Results:
- Successful separation of various urinary components was achieved.
- The new stationary phase demonstrated effectiveness in complex sample matrices.
- High sensitivity was observed with both ECD and LIFD.
Conclusions:
- The developed cross-linked polymer stationary phase is suitable for capillary chromatography.
- This approach enhances the separation and detection of urinary analytes.
- The method offers a promising tool for clinical and biochemical analysis.
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