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Sample work-up by column techniques
1Department of Physiological Chemistry and Department of Clinical Chemistry, Karolinska Institutet, S-104 01 Stockholm, Sweden.
Journal of Pharmaceutical and Biomedical Analysis
|January 1, 1984
Summary
This review covers extraction and purification methods for biological samples using dextran gels. It highlights applications for isolating various metabolites and steroids from biological fluids and tissues.
Area of Science:
- Biochemistry
- Analytical Chemistry
- Chromatography
Background:
- Biological sample preparation is crucial for accurate analysis.
- Traditional methods can be time-consuming and less efficient.
- Novel extraction and purification techniques are needed for complex biological matrices.
Purpose of the Study:
- To review advanced filtration techniques for biological sample purification.
- To discuss the application of lipophilic neutral and ion-exchange dextran gels.
- To highlight selective isolation procedures for various biomolecules.
Main Methods:
- Filtration through lipophilic neutral dextran gels.
- Ion-exchange dextran gel filtration.
- Liquid-gel extraction.
- Reversed-phase liquid chromatography.
- Ligand-exchange chromatography.
Main Results:
- Sephadex and Lipidex gels offer effective extraction from biological fluids and solid samples.
- Selective isolation of estrogens, lignans, isoflavones, and steroids is achievable.
- Metabolite extraction efficiency is enhanced by these methods.
Conclusions:
- Dextran gel filtration provides a versatile approach for biological sample purification.
- These methods are applicable to a wide range of metabolites and steroids.
- Advanced chromatographic techniques complement gel filtration for enhanced selectivity.