Related Experiment Video
Updated: Aug 6, 2026

Preparation and In Vivo Use of an Activity-based Probe for N-acylethanolamine Acid Amidase
Published on: November 23, 2016
Analysis of the 1,4-benzodiazepines by methods based on hydrolysis
1Department of Physical Chemistry, Faculty of Pharmacy, Charles University, 501 65 Hradec Králové, Czechoslovakia.
Abstract:
The analytical applications of the hydrolysis of 1,4-benzodiazepines to the corresponding benzophenone derivatives are reviewed according to the analytical methods used for their final determination. The scope and limitations of the individual methods for the hydrolysis products are discussed: Colour reactions, photometry, fluorimetry, thin-layer chromatography, gas chromatography and high-performance liquid chromatography. Published data on the kinetic parameters and mechanisms are summarized in an attempt to explain the problems involved in exploiting this reaction for analytical applications.
Related Concept Videos
NMR Spectroscopy of Benzene Derivatives
Diazonium Group Substitution: –OH and –H
Hydrolysis of Chlorobenzene to Phenol: Dow Process
Phase I Reactions: Hydrolytic Reactions
An important hydrolytic reaction is ester hydrolysis. Ester bonds, often found in prodrugs, are broken down, increasing the solubility of drugs like aspirin and lidocaine for more straightforward elimination. Amide hydrolysis is another critical reaction, targeting amide bonds prevalent...
Structure of Benzene: Kekulé Model
He proposed that benzene has a cyclic structure of six carbon atoms attached to one hydrogen atom each, with three alternating pi bonds.
Basicity of Heterocyclic Aromatic Amines
