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Plasma assay for the antineoplastic agent VP 16-213 (etoposide) using high-performance liquid chromatography with

J J Holthuis1, F M Römkens, H M Pinedo

  • 1Pharmaceutical Laboratory, State University of Utrecht, Catharijnesingel 60, 3511 GH Utrecht, The Netherlands.

Journal of Pharmaceutical and Biomedical Analysis
|January 1, 1983
PubMed

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Effects of EDTA on End-Point Detection Methods01:18

Effects of EDTA on End-Point Detection Methods

Different methods, such as visual observance of metal-ion indicators, spectroscopic techniques, and potentiometric methods, can determine the endpoint of an EDTA titration.
In the visual method, metal-ion indicators (metallochromic dyes), which have distinct colors in their free and complex forms, are added to the mixture to signal the titration's end point. They form stable complexes with metal ions, but these complexes are weaker than the corresponding metal–EDTA complexes. As a result, EDTA...