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Updated: Aug 6, 2026
Interphase Fluorescence in situ Hybridization of Bone Marrow Smears of Multiple Myeloma
Published on: April 15, 2022
Evaluation of c-MYC status in primary acquired cholesteatoma by using fluorescence in situ hybridization technique
Kayhan Ozturk1, Mahmut Selman Yildirim, Hasan Acar
1Department of Otolaryngology, Meram Medical Faculty, Selçuk University, Konya, Turkey. kayhanozturk@selcuk.edu.tr
Objective:
The object of study was to investigate the status of c-MYC oncogene in primary acquired cholesteatoma.
Study Design:
Descriptive study.
Methods:
Cholesteatoma samples were obtained from 15 patients with primary acquired cholesteatoma during surgical operation. Fluorescence in situ hybridization with a mixed DNA probe, which is specific for c-MYC located on 8q24 and chromosome 8 specific-alpha-satellite DNA probe (dual color), was used on the interphase nuclei.
Results:
Copy number of c-MYC oncogene and aneuploidy of chromosome 8 were 21.2% +/- 14.4% and 21.7% +/- 14.8%, respectively. There was no significant difference between copy number of c-MYC and frequency of chromosome 8 aneuploidy (p > 0.05). Ten of 15 cases showed different percentage of c-MYC and chromosome 8 aneuploidy, whereas 5 (33.3%) of 15 cases showed a normal distribution of c-MYC and chromosome 8 signals.
Conclusion:
The copy number of c-MYC in 10 of 15 cases was found to be high as observed for chromosome 8 aneuploidy in primary acquired cholesteatoma. These findings suggest that the ability of hyperproliferation of primary acquired cholesteatoma might have been related to c-MYC copy number by deregulating c-MYC expression.
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