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Updated: Aug 6, 2026

Assaying Protein Kinase Activity with Radiolabeled ATP
Published on: May 26, 2017
BRSK2 is activated by cyclic AMP-dependent protein kinase A through phosphorylation at Thr260
Zekun Guo1, Wenwen Tang, Jian Yuan
1State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, Shanghai 200433, PR China.
Abstract:
Brain selective kinase 2 (BRSK2) has been identified as a member of AMPK related kinases. LKB1 can phosphorylate the Thr174 of BRSK2, increasing its activity >50-fold. In this study, we identified cAMP-dependent protein kinase A (PKA) as another upstream kinase of BRSK2, which can phosphorylate BRSK2 at Thr260. The association between these two proteins was confirmed by GST pull-down. Furthermore, our study indicated that the kinase activity of BRSK2 can be increased through phosphorylation by PKA.
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