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Updated: Aug 6, 2026
Anaerobic Growth and Maintenance of Mammalian Cell Lines
Published on: July 21, 2018
cAMP controls oxygen metabolism in mammalian cells
Claudia Piccoli1, Salvatore Scacco, Francesco Bellomo
1Department of Biomedical Science, University of Foggia, Foggia, Italy.
Abstract:
The impact of cAMP on ROS-balance in human and mammalian cell cultures was studied. cAMP reduced accumulation of ROS induced by serum-limitation, under conditions in which there was no significant change in the activity of scavenger systems. This effect was associated with cAMP-dependent activation of the NADH-ubiquinone oxidoreductase activity of complex I. In fibroblasts from a patient a genetic defect in the 75 kDa FeS-protein subunit of complex I resulted in inhibition of the activity of the complex and enhanced ROS production, which were reversed by cAMP. A missense genetic defect in the NDUFS4 subunit, putative substrate of PKA, suppressed, on the other hand, the activity of the complex and prevented ROS production.
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