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Published on: May 8, 2013
[Haemolytic and proteolytic properties of Serratia spp. rods]
Beata Ulatowska1, Eugenia Gospodarek
1Katedra i Zakład Mikrobiologii Collegium Medicum im. Ludwika Rydygiera w Bydgoszczy, Uniwersytet Mikołaja Kopernika w Toruniu.
Abstract:
For over fifty years Serratia spp. rods have increasingly been isolated from clinical specimens. The isolation has arisen mainly from hospitalised patients. The development of Serratia spp. infections is associated with predisposing factors of patients, occurrence in both natural settings and within the hospital environment and the virulence factors of bacteria. CDM medium with casein sodium salt, gelatine and Skim Milk were used to detect proteolytic properties. Over 95% of analysed strains produced gelatinase. Casein sodium salt was hydrolysed by 89,6% to 93,1% of strains depending on cultivation temperature. Skim Milk was hydrolysed by 78,5% to 84,7% of analysed Serratia spp. strains. Haemolytic properties of Serratia spp. rods were checked on a solid medium with human and sheep red blood cells at 22, 30, and 37 degrees C. The haemolysis of human erythrocytes at 37 degrees C was detected most frequently at 38,2%. There were no significant differences identified when cultivation temperature was observed.
Insights
Serratia species, often found in clinical settings, exhibit significant proteolytic and hemolytic activities. These bacterial virulence factors are crucial for understanding their role in hospital-acquired infections.
Area of Science:
- Microbiology
- Clinical Bacteriology
Context:
- Serratia species are increasingly isolated from clinical specimens, particularly in hospital settings.
- Patient predisposing factors, environmental presence, and bacterial virulence contribute to Serratia infections.
Purpose:
- To investigate the proteolytic and hemolytic properties of Serratia species.
- To assess the correlation between these virulence factors and clinical isolates.
Summary:
- CDM medium was used to test casein sodium salt, gelatin, and skim milk hydrolysis.
- Over 95% of strains produced gelatinase, with high percentages hydrolyzing casein and skim milk.
- Hemolytic activity was tested on human and sheep erythrocytes at various temperatures, with 38.2% showing human erythrocyte hemolysis at 37°C.
Impact:
- Understanding Serratia virulence factors aids in infection control strategies.
- Highlights the importance of proteolytic and hemolytic assays in characterizing clinical isolates.
- Provides data on Serratia spp. properties relevant to healthcare-associated infections.
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