Triterpenes from natural and transformed roots of Plocama pendula

Plocama pendula root cultures transformed by Agrobacterium rhizogenes were established. From these cultures two new pentacyclic triterpenes, 3-epi-pomolic acid 3alpha-acetate (3alpha-acetoxy-19alpha-hydroxyursa-12-en-28-oic acid) (1) and baloic acid (3alpha-acetoxy-19alpha-hydroxyursa-12,20(30)-dien-28-oic acid) (9), were isolated. The former was also obtained from P. pendula natural roots, together with another two new pentacyclic triterpenes, 3-epi-pomolic acid (3alpha,19alpha-dihydroxyursa-12-en-28-oic acid) (3) and 19alpha-hydroxyoleanonic acid (19alpha-hydroxy-3-oxo-olean-12-en-28-oic acid) (12).