Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Alternative splicing in human transcriptome: functional and structural influence on proteins.

Kei Yura1, Masafumi Shionyu, Kei Hagino

  • 1Quantum Bioinformatics Team, Center for Computational Science and Engineering, Japan Atomic Energy Agency, 8-1 Umemidai, Kizu-cho, Souraku-gun, Kyoto 619-0215, Japan.

Gene
|July 29, 2006
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Early steroid initiation may improve outcomes in immune checkpoint inhibitor-related cholangitis: a multicenter cohort study.

Immunological medicine·2026
Same author

A PARK9 iPSC-Derived Dopaminergic Neuron Model Enables Drug Screening Targeting Autophagy-Lysosome Pathway Dysfunction in Parkinson's Disease.

Journal of neurochemistry·2026
Same author

Temporal orchestration of transcriptional and epigenomic programming underlying maternal embryonic diapause in a cricket model.

Communications biology·2026
Same author

Residue interaction chains facilitating the structural change of ND6 subunit in respiratory complex I.

Biochemical and biophysical research communications·2026
Same author

Suppression of ATM kinase signaling accelerates cellular senescence.

Stem cell reports·2026
Same author

Re-examination of the morphological and molecular features of <i>Amanita clarisquamosa</i> and <i>Amanita avellaneosquamosa</i> based on specimens collected in Hokkaido.

Mycoscience·2026
Same journal

TBX6 promotes proliferation, invasion, and migration in colorectal cancer: Integrated transcriptomic and protein interaction network analysis.

Gene·2026
Same journal

Face/off: phase-specific modeling of lineage plasticity using near-patient models in genitourinary cancers.

Gene·2026
Same journal

Hierarchical analysis of metabolic phenotype reveals distinct microbiota and circulatory transcriptome in metabolic dysfunction-associated steatotic liver disease.

Gene·2026
Same journal

Mutation T71R enhanced the structural stability and functional activity of wild type superoxide dismutase cloned from soil metagenome.

Gene·2026
Same journal

Reduced ATXN1 expression as an adverse prognostic indicator in Acute myeloid leukemia.

Gene·2026
Same journal

Constructing regulatory networks of Rubisco post-translational modifications: a novel avenue for engineering environment adaptive plants.

Gene·2026
See all related articles
This summary is machine-generated.

Alternative splicing generates protein diversity from single genes. This study reveals it significantly alters protein structure and function, impacting protein networks through conformational changes.

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Alternative splicing is a key mechanism for generating proteomic diversity from a limited genome.
  • It allows a single gene to produce multiple protein isoforms with potentially different functions.
  • Understanding the structural and functional impact of alternative splicing is crucial for comprehending gene regulation and cellular processes.

Purpose of the Study:

  • To analyze how alternative splicing generates protein structural and functional variety.
  • To evaluate the impact of alternatively spliced regions on protein domains, interaction sites, and overall protein conformation.
  • To explore the role of alternative splicing in modulating protein networks.

Main Methods:

  • Analysis of human full-length cDNAs, assuming all alternatively spliced mRNAs are translated into proteins.

Related Experiment Videos

  • Evaluation of the lengths of alternatively spliced amino acid sequences relative to average protein domain size.
  • Comprehensive assessment of presumptive three-dimensional structures of alternatively spliced products.

    • Main Results:

    • Alternatively spliced amino acid sequences are often shorter than average protein domains.
    • Over half of the alternatively spliced products are involved in signal transduction, transcription, and translation.
    • More than half of alternatively spliced regions function as protein interaction sites, and 67% significantly alter protein structural cores, leading to conformational changes.

    Conclusions:

    • Alternative splicing significantly diversifies protein structure and function.
    • It plays a critical role in shaping protein interaction networks by inducing substantial conformational alterations.
    • Alternative splicing is a key regulator of cellular processes through modulation of protein conformation and interactions.