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Updated: Aug 6, 2026

Production and Visualization of Bacterial Spheroplasts and Protoplasts to Characterize Antimicrobial Peptide Localization
Published on: August 11, 2018
Peptide-PEG amphiphiles as cytophobic coatings for mammalian and bacterial cells
Daniel J Kenan1, Elisabeth B Walsh, Steven R Meyers
1Department of Pathology, Duke University Medical Center, Durham, North Carolina 27710, USA. kenan001@mc.duke.edu
Abstract:
Amphiphilic macromolecules containing a polystyrene-adherent peptide domain and a cell-repellent poly(ethylene glycol) domain were designed, synthesized, and evaluated as a cytophobic surface coating. Such cytophobic, or cell-repellent, coatings are of interest for varied medical and biotechnological applications. The composition of the polystyrene binding peptide domain was identified using an M13 phage display library. ELISA and atomic force spectroscopy were used to evaluate the binding affinity of the amphiphile peptide domain to polystyrene. When coated onto polystyrene, the amphiphile reduced cell adhesion of two distinct mammalian cell lines and pathogenic Staphylococcus aureus strains.
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