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Updated: Aug 6, 2026

Monitoring Stub1-Mediated Pexophagy
Published on: May 12, 2023
The Hansenula polymorpha ATG25 gene encodes a novel coiled-coil protein that is required for macropexophagy
Iryna Monastyrska1, Jan A K W Kiel, Arjen M Krikken
1Eukaryotic Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, The Netherlands.
Abstract:
We have isolated the Hansenula polymorpha ATG25 gene, which is required for glucose-induced selective peroxisome degradation by macropexophagy. ATG25 represents a novel gene that encodes a 45 kDa coiled-coil protein. We show that this protein colocalizes with Atg11 on a small structure, which most likely represents the pre-autophagosomal structure (PAS). In cells of a constructed ATG25 deletion strain (atg25) peroxisomes are constitutively degraded by nonselective microautophagy, a process that in WT H. polymorpha is only observed at nitrogen limitation conditions. This suggests that nonselective microautophagy is deregulated in H. polymorpha atg25 cells.
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