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Updated: Aug 6, 2026

Bacterial Detection & Identification Using Electrochemical Sensors
Published on: April 23, 2013
Multiplexed analyte and oligonucleotide detection on microarrays using several redox enzymes in conjunction with
Kilian Dill1, Andrey Ghindilis, Kevin Schwarzkopf
1CombiMatrix Corporation, 6500 Harbour Heights Parkway, Suite 301, Mukilteo, WA 98275, USA. kilian@combimatrix.com
Abstract:
We show that multiple enzyme tags may be used in an immunoassay format or for the detection of sequence-specific DNA on microarrays. The assays may be multiplexed and monitored under separate solution and voltage differences. Thus, the detection method relies on an electrochemical detection format, whereby multiple enzymes can be sensed. In our case we utilize horseradish peroxidase, laccase, and glucose dehydrogenase as enzymes attached to specific antibodies or to streptavidin.
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There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or quantified.

