Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The cysteine string protein multimeric complex.

Leigh Anne Swayne1, Katy E Beck, Janice E A Braun

  • 1Department of Physiology and Biophysics, Hotchkiss Brain Institute, University of Calgary, Calgary, Alberta, Canada T2N 4N1.

Biochemical and Biophysical Research Communications
|August 1, 2006
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Early Surge in Brain GFAP Distribution in Female Hamsters With Mild Peripheral COVID-19.

Journal of neurochemistry·2026
Same author

J-domain proteins: from molecular mechanisms to diseases.

Cell stress & chaperones·2025
Same author

ATP increases murine neuroblastoma cell size through a PANX1- and macropinocytosis-dependent mechanism.

Biology open·2025
Same author

Quantification of morphological, functional, and biochemical features of H9c2 rat cardiomyoblast retinoic acid differentiation.

Journal of molecular and cellular cardiology plus·2025
Same author

Dual role for pannexin 1 at synapses: regulating functional and morphological plasticity.

The Journal of physiology·2024
Same author

J-domain proteins: From molecular mechanisms to diseases.

Cell stress & chaperones·2024

Cysteine string protein alpha (CSPalpha) interacts with syntaxin 1A and N-type channels, influencing synaptic transmission. Huntingtin exon 1 disrupts these interactions, offering insights into protein complex roles in neurons.

Area of Science:

  • Neuroscience
  • Cell Biology
  • Molecular Biology

Background:

  • Cysteine string protein alpha (CSPalpha) is involved in cellular folding and located on secretory vesicles.
  • CSPalpha, Hsc70, and SGT form a guanine nucleotide exchange factor (GEF) for G(alphas).
  • This complex associates with N-type calcium channels, inhibiting them and impacting synaptic vesicle exocytosis.

Purpose of the Study:

  • To investigate the role of syntaxin 1A in the CSPalpha/G protein/N-type calcium channel complex.
  • To determine the interaction sites of syntaxin 1A within this complex.
  • To explore the effect of huntingtin exon 1 on the complex.

Main Methods:

  • Co-immunoprecipitation to identify protein interactions.
  • Demonstration of protein displacement using specific fragments.

Related Experiment Videos

  • Analysis of protein complex formation in neuronal systems.

    • Main Results:

    • Syntaxin 1A directly interacts with CSPalpha, Hsc70, and the synprint region of N-type channels.
    • Huntingtin exon 1 was shown to displace both syntaxin 1A and CSPalpha from N-type channels.
    • Syntaxin 1A is a component of the CSPalpha/GEF system regulating N-type calcium channels.

    Conclusions:

    • Syntaxin 1A is a key component of the CSPalpha/GEF complex that modulates N-type calcium channel activity.
    • The interaction of syntaxin 1A with the complex is sensitive to huntingtin exon 1.
    • Further research into these protein interactions is crucial for understanding synaptic transmission regulation.