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Updated: Aug 6, 2026
11:16
A Microfluidic Chip for ICPMS Sample Introduction
Published on: March 5, 2015
Reversed-phase liquid chromatography on a microchip with sample injector and monolithic silica column
Akihiko Ishida1, Takahiro Yoshikawa, Masamichi Natsume
1Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, Nishi 8, Kita 13, Sapporo 060-8628, Japan. ishida-a@eng.hokudai.ac.jp <ishida-a@eng.hokudai.ac.jp>
Journal of Chromatography. A
|August 1, 2006
Summary
This study introduces a novel microchip for reversed-phase liquid chromatography (LC) using porous monolithic silica. The developed microchip achieved high separation efficiencies for catechins, advancing microchip LC technology.
Area of Science:
- Analytical Chemistry
- Microfluidics
- Separation Science
Background:
- Micro total analysis systems (µTAS) rely on pressure-driven liquid chromatography (LC) as a key analytical component.
- Research on microchip-based LC (µLC) remains limited, presenting an opportunity for technological advancement.
Purpose of the Study:
- To develop and characterize a novel microchip for reversed-phase LC.
- To investigate the impact of channel geometry on separation performance in µLC.
Main Methods:
- Fabrication of a microchip featuring a double T-shaped injector and a serpentine separation channel.
- In-situ preparation of octadecyl-modified porous monolithic silica using a sol-gel process.
- Evaluation of band dispersion at channel turns under pressure-driven flow.
Main Results:
- High separation efficiencies, ranging from 15,000 to 18,000 plates/m, were achieved for catechin analysis.
- The study examined the influence of turn geometry on band dispersion within the microchannel.
Conclusions:
- The developed microchip demonstrates high separation efficiency for µLC applications.
- This work contributes to the advancement of microchip liquid chromatography technology.
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