Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Retinal oximetry using intravitreal illumination.

David A Salyer1, Neil Beaudry, Sreenivasa Basavanthappa

  • 1Optical Sciences Center, University of Arizona, Tucson, Arizona 85721, USA. salyer@optics.arizona.edu

Current Eye Research
|August 1, 2006
PubMed
Summary

This study demonstrates intravitreal illumination for retinal oximetry in swine, improving accuracy by reducing vessel glint. Measurements showed lower venous oxygen saturation after vitrectomy, highlighting a potential research application.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Azithromycin for Preschoolers with Wheezing in the Emergency Department.

The New England journal of medicine·2026
Same author

Structure and thermorheological properties of TAG-estolide lipid macromolecules from Orychophragmus violaceus seeds.

International journal of biological macromolecules·2025
Same author

EMS Treatment Guidelines in Major Traumatic Brain Injury With Positive Pressure Ventilation.

JAMA surgery·2024
Same author

Tribocatalytically-active nickel/cobalt phosphorous films for universal protection in a hydrocarbon-rich environment.

Scientific reports·2023
Same author

Correlation between prehospital and in-hospital hypotension and outcomes after traumatic brain injury.

The American journal of emergency medicine·2023
Same author

Dual-band wide-angle perfect absorber based on the relative displacement of graphene nanoribbons in the mid-infrared range.

Optics express·2022

Area of Science:

  • Ophthalmology
  • Biomedical Engineering
  • Physiology

Background:

  • Retinal oximetry measures oxygen saturation in retinal vessels.
  • Transcorneal illumination methods face challenges like vessel glint.
  • Pars plana vitrectomy alters the intraocular environment.

Purpose of the Study:

  • To demonstrate spectroscopic retinal oximetry using intravitreal illumination in swine.
  • To measure retinal arterial and venous oxygen saturations under varying inspired oxygen levels.
  • To assess the utility of intravitreal illumination for overcoming limitations of transcorneal methods.

Main Methods:

  • Pars plana vitrectomy was performed on swine.
  • Intravitreal fiberoptic illumination was used to image retinal vasculature.

Related Experiment Videos

  • Multispectral images were acquired as arterial oxygen saturation was modulated.
  • Spectra were analyzed to calculate retinal vessel oxygen saturation.

    • Main Results:

    • Intravitreal illumination significantly reduced vessel glint, simplifying spectral analysis.
    • Reduced glint enhanced accuracy in measuring spectral optical density.
    • Abnormally low retinal venous oxygen saturations were observed post-vitrectomy.

    Conclusions:

    • Spectroscopic retinal oximetry with intravitreal illumination is feasible.
    • This technique offers advantages over transcorneal illumination for research purposes.
    • Intravitreal illumination facilitates accurate retinal oximetry in challenging conditions.