Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A functional interaction between sprouty proteins and caveolin-1.

Miguel A Cabrita1, Fabienne Jäggi, Sandra P Widjaja

  • 1Institute of Biochemistry and Genetics, Department of Clinical-Biological Sciences, Center of Biomedicine, University of Basel, Mattenstrasse 28, 4058 Basel, Switzerland.

The Journal of Biological Chemistry
|August 1, 2006
PubMed
Summary

All four Sprouty proteins interact with Caveolin-1, modulating growth factor signaling pathways. This interaction

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Protocol to screen for Sorafenib resistance regulators using pooled lentiviral shRNA library and a Sorafenib-resistant hepatocellular carcinoma cell model.

STAR protocols·2023
Same author

Tracking and characterization of partial and full epithelial-mesenchymal transition cells in a mouse model of metastatic breast cancer.

STAR protocols·2022
Same author

Multiple roles for Bcl-3 in mammary gland branching, stromal collagen invasion, involution and tumor pathology.

Breast cancer research : BCR·2022
Same author

Distinct contributions of partial and full EMT to breast cancer malignancy.

Developmental cell·2021
Same author

Hierarchy of TGFβ/SMAD, Hippo/YAP/TAZ, and Wnt/β-catenin signaling in melanoma phenotype switching.

Life science alliance·2021
Same author

YAP/TAZ and ATF4 drive resistance to Sorafenib in hepatocellular carcinoma by preventing ferroptosis.

EMBO molecular medicine·2021

Area of Science:

  • Cellular biology
  • Molecular signaling
  • Protein interactions

Background:

  • Sprouty proteins regulate growth factor signaling.
  • Caveolin-1 is a key structural protein in caveolae.
  • Sprouty-1 and Sprouty-2 associate with Caveolin-1.

Purpose of the Study:

  • To investigate the interaction between all four mammalian Sprouty family members and Caveolin-1.
  • To determine the binding sites and functional consequences of the Sprouty-Caveolin-1 interaction.
  • To explore the specificity of this interaction in modulating growth factor signaling.

Main Methods:

  • Co-immunoprecipitation assays to confirm physical interactions.
  • Site-directed mutagenesis to identify binding domains.
  • Analysis of mitogen-activated protein kinase (MAPK) activation under various conditions.

Related Experiment Videos

Main Results:

  • All four Sprouty proteins physically interact with Caveolin-1.
  • The C-terminal domain of Sprouty and the C terminus of Caveolin-1 are crucial for binding.
  • Sprouty isoform-specific differences in cooperating with Caveolin-1 to inhibit MAPK activation were observed.
  • The modulation of signaling is dependent on the growth factor and cell density.

Conclusions:

  • The Sprouty-Caveolin-1 interaction is a significant mechanism for regulating growth factor signaling.
  • This interaction exhibits specificity based on Sprouty isoform, growth factor, and cellular context.
  • Findings provide insights into the complex interplay of signaling modulators in cellular pathways.