Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

DNA Isolation

DNA Isolation

DNA from cells is required for many biotechnology and research applications, such as molecular cloning. To remove and purify DNA from cells, researchers use various methods of DNA extraction. While the specifics of different protocols may vary, some general concepts underlie the process of DNA extraction.

DNA Isolation

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Population structure and demographic history for year cohort dynamics of landlocked ayu Plecoglossus altivelis altivelis in dam reservoir of Japan.

Environmental science and pollution research international·2024

Same author

Stock assessment of landlocked ayu Plecoglossus altivelis altivelis in Japan through length-based models.

Environmental science and pollution research international·2022

Same author

Stochastic optimal switching model for migrating population dynamics.

Journal of biological dynamics·2019

Same author

A novel mitochondrial intergenic spacer reflecting population structure of Pacific oyster.

Journal of applied genetics·2006

Same author

Molecular identification of hairtail species (Pisces: Trichiuridae) based on PCR-RFLP analysis of the mitochondrial 16S rRNA gene.

Journal of applied genetics·2005

Same author

Rapid PCR-RFLP method for discrimination of imported and domestic mackerel.

Marine biotechnology (New York, N.Y.)·2005

Search research articles

Related Experiment Video

Updated: Jun 30, 2026

A Noninvasive Hair Sampling Technique to Obtain High Quality DNA from Elusive Small Mammals

A Noninvasive Hair Sampling Technique to Obtain High Quality DNA from Elusive Small Mammals

Published on: March 13, 2011

A simple and reliable method for DNA extraction from bivalve mantle.

Futoshi Aranishi¹, Takane Okimoto

¹Department of Biological and Environmental Sciences, Miyazaki University, Miyazaki 889-2192, Japan. aranishi@cc.miyazaki-u.ac.jp

Journal of Applied Genetics

|August 1, 2006

Summary

A new method efficiently extracts genomic DNA from Pacific oyster (Crassostrea gigas) tissues, including preserved samples. This DNA is suitable for PCR amplification, aiding population structure analysis.

More Related Videos

Simultaneous DNA-RNA Extraction from Coastal Sediments and Quantification of 16S rRNA Genes and Transcripts by Real-time PCR

Simultaneous DNA-RNA Extraction from Coastal Sediments and Quantification of 16S rRNA Genes and Transcripts by Real-time PCR

Published on: June 11, 2016

The Barnacle Balanus improvisus as a Marine Model - Culturing and Gene Expression

The Barnacle Balanus improvisus as a Marine Model - Culturing and Gene Expression

Published on: August 8, 2018

Related Experiment Videos

Last Updated: Jun 30, 2026

A Noninvasive Hair Sampling Technique to Obtain High Quality DNA from Elusive Small Mammals

A Noninvasive Hair Sampling Technique to Obtain High Quality DNA from Elusive Small Mammals

Published on: March 13, 2011

Simultaneous DNA-RNA Extraction from Coastal Sediments and Quantification of 16S rRNA Genes and Transcripts by Real-time PCR

Simultaneous DNA-RNA Extraction from Coastal Sediments and Quantification of 16S rRNA Genes and Transcripts by Real-time PCR

Published on: June 11, 2016

The Barnacle Balanus improvisus as a Marine Model - Culturing and Gene Expression

The Barnacle Balanus improvisus as a Marine Model - Culturing and Gene Expression

Published on: August 8, 2018

Area of Science:

Marine Biology
Genetics
Biotechnology

Background:

Pacific oysters (Crassostrea gigas) are ecologically and economically important bivalves.
Accurate population structure analysis requires high-quality genomic DNA.
Existing DNA extraction methods can be inefficient or unsuitable for preserved field samples.

Purpose of the Study:

To develop a simple, reliable, and economical method for extracting genomic DNA from preserved Pacific oyster mantle tissues.
To ensure the extracted DNA is suitable for reproducible Polymerase Chain Reaction (PCR) amplification.
To facilitate population genetics studies using field-collected bivalve specimens.

Main Methods:

Tissue destruction using Proteinase K, Chelex 100 resin, detergents, and urea.
Preferential capturing of genomic DNA using silica particles.
Application to fresh, frozen, and ethanol-preserved mantle tissues.

Main Results:

A simple and reliable DNA extraction method was established for Crassostrea gigas.
Approximately 5 mg of mantle tissue yielded sufficient DNA for hundreds of PCR reactions.
The method is effective for amplifying the hypervariable mitochondrial DNA intergenic spacer, a key population genetics marker.
The protocol works with fresh, frozen, and ethanol-preserved tissues.

Conclusions:

The developed method provides high-quality genomic DNA from various preserved oyster tissues.
This rapid and economical technique is ideal for large-scale population structure analysis of bivalves.
It enables genetic studies on specimens collected in the field and preserved at ambient temperatures.