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Applications of automated amino acid analysis using 9-fluorenylmethyl chloroformate
P A Haynes1, D Sheumack, L G Greig
1School of Chemistry, Macquarie University, NSW, Australia.
Journal of Chromatography
|December 27, 1991
Abstract:
A rapid and sensitive fully automated method for the determination of primary and secondary amino acids in different matrices is described. Amino acids are derivatized with 9-fluorenylmethyl chloroformate using an automated precolumn derivatization technique. Data are presented to show that the technique is both reproducible and highly sensitive. Applications of the technique are presented, including the analysis of peptide and protein hydrolysates and the profiling of free amino acids in physiological fluids.