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A Yeast 2-Hybrid Screen in Batch to Compare Protein Interactions
Published on: June 6, 2018
Analysis of PDZ domain interactions using yeast two-hybrid and coimmunoprecipitation assays
Hyun Woo Lee1, Jaewon Ko, Eunjoon Kim
1Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon.
Methods in Molecular Biology (Clifton, N.J.)
|August 2, 2006
Abstract:
The PDZ domain is a protein-protein interaction module that interacts with a C-terminal short peptide motif in its binding partners. A variety of methods have been used to study PDZ domain interactions. This chapter details the two methods most commonly used in the analysis of PDZ interactions: yeast two-hybrid and coimmunoprecipitation assays. In addition, we discuss the features that must be considered for an efficient analysis of PDZ interactions.
