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In Vitro Analysis of PDZ-dependent CFTR Macromolecular Signaling Complexes
Published on: August 13, 2012
Clustering assay for studying the interaction of membrane proteins with PDZ domain proteins
1Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon.
Methods in Molecular Biology (Clifton, N.J.)
|August 2, 2006
Abstract:
Some membrane proteins must be clustered at target sites to efficiently perform their functions. PDZ domain-containing scaffold proteins bind to the tails of target membrane proteins and promote their localization and clustering on the cell surface. This chapter describes the experimental details of the clustering assay, using the interaction between potassium channels and PSD-95, an abundant PDZ domain protein in neuronal synapses, as a model.
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