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Solution-phase surface modification in intact poly(dimethylsiloxane) microfluidic channels.

Guodong Sui1, Jinyi Wang, Chung-Cheng Lee

  • 1Crump Institute for Molecular Imaging and Department of Molecular & Medical Pharmacology, David Geffen School of Medicine at UCLA, 700 Westwood Plaza, Los Angeles, California 90095, USA.

Analytical Chemistry
|August 2, 2006
PubMed
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This study presents a new surface modification method for poly(dimethylsiloxane) (PDMS) microfluidic devices. The improved approach enhances PDMS surfaces for biomolecule attachment and protein resistance, enabling advanced biological applications.

Area of Science:

  • Materials Science
  • Surface Chemistry
  • Biotechnology

Background:

  • Poly(dimethylsiloxane) (PDMS) is widely used in microfluidic devices.
  • Surface modification of PDMS is crucial for specific applications, such as reducing nonspecific protein adsorption and enabling biomolecule immobilization.
  • Existing methods can be complex or require device disassembly.

Purpose of the Study:

  • To develop a simple, solution-phase surface modification method for intact PDMS microfluidic devices.
  • To introduce poly(ethylene glycol) (PEG) for protein passivation and amine (NH2) groups for biomolecule attachment.
  • To evaluate the stability and functionality of the modified PDMS surfaces.

Main Methods:

  • An improved approach involving sequential oxidation in acidic H2O2 and silanization with neat silane reagents.

Related Experiment Videos

  • Surface characterization using X-ray electron spectroscopy and temporal contact angle measurements.
  • Functional testing by introducing fluorescent protein solutions and assessing protein repelling characteristics in PEG-grafted channels.

    • Main Results:

    • Successful introduction of PEG and NH2 functional groups onto PDMS surfaces.
    • PEG-grafted PDMS surfaces demonstrated improved short-term surface dynamics and robust long-term stability.
    • Amino-grafted PDMS microchannels showed stability and potential for further modification with peptides, DNA, and proteins.

    Conclusions:

    • The developed method offers a convenient approach for modifying PDMS microfluidic devices without postassembly.
    • The functionalized PDMS surfaces are suitable for various applications, including cell immobilization, DNA hybridization, and immunoassays.
    • This technique enhances the utility of PDMS in routine analytical chemistry and biology laboratory applications.